Lecture 1 - Theory of absorption of light by molecules: propagation of an electromagnetic wave through space - I
Lecture 2 - Theory of absorption of light by molecules: propagation of an electromagnetic wave through space - II
Lecture 3 - Jablonski diagram - I
Lecture 4 - Jablonski diagram - II
Lecture 5 - Instrumentation, Beer's law and the extinction coefficient and Chromophore: factors affecting the absorption properties of a chromophore
Lecture 6 - Solvent-induced heterogeneity, red-shifts and blue shifts; polarity vs polarizability of the medium - I
Lecture 7 - Solvent-induced heterogeneity, red-shifts and blue shifts; polarity vs polarizability of the medium - II
Lecture 8 - Spectral shifts in rhodopsin and vision
Lecture 9 - Structural studies DNA using absorption spectroscopy Helix coil transition of double stranded DNA
Lecture 10 - Melting curve of DNA: Hyperchromicity and hypochromicity
Lecture 11 - Structural studies of proteins using absorption spectroscopy
Lecture 12 - Practical Considerations: Spectral bandwidth, wavelength error and stray light
Lecture 13 - Structural studies of proteins using absorption spectroscopy
Lecture 14 - Theory of fluorescence spectroscopy - I
Lecture 15 - Photoluminescence, emission, and absorption Stokes Shift
Lecture 16 - Theory of fluorescence spectroscopy - II
Lecture 17 - Advanced Application of Flourescence Spectroscopy
Lecture 18 - Solvent and Environmental Effects on Fluorescence
Lecture 19 - Inner Filter Effect in Fluorescence
Lecture 20 - Flourescence Lifetime
Lecture 21 - Fluorescence resonance energy transfer
Lecture 22 - Introduction to InfraRed Spectroscopy
Lecture 23 - Introduction to Vibrational Spectroscopy: Infrared Spectroscopy
Lecture 24 - Fundamerntal of FT-IR
Lecture 25 - Introduction to Raman Spectroscopy
Lecture 26 - Fundamental and Application of Raman Spectroscopy
Lecture 27 - Biological application of FT-IR
Lecture 28 - Introduction Linearly and circularly polarized light
Lecture 29 - Instrumentation of Circular Dichroism (CD) Spectroscopy
Lecture 30 - Experimental: sample concentration and cell pathlength Solvent effects
Lecture 31 - Characteristics of CD and ORD (optical rotatory dispersion) spectroscopy: Cotton Effect
Lecture 32 - Identification of known optical isomers
Lecture 33 - Determination of protein secondary structure
Lecture 34 - Detection of molten globule like structure of proteins
Lecture 35 - Applications of CD spectroscopy in studying amyloid based neurodegenerative disorders
Lecture 36 - Determination of nucleic acid conformations
Lecture 37 - Use of CD spectroscopy to study stacking orientation of the base pairs
Lecture 38 - Application of Spectroscopy in Biopharma Setting - I
Lecture 39 - Application of Spectroscopy in Biopharma Setting - II
Lecture 40 - Application of Raman Spectroscopy in Pharma and Biopharma Industry - I
Lecture 41 - Application of Raman Spectroscopy in Pharma and Biopharma Industry - II
Lecture 42 - Fluorescence Spectroscopy Application for various Pharmaceutical products
